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LABORATORY MANUAL FOR GENETIC ENGINEERING

LABORATORY MANUAL FOR GENETIC ENGINEERING

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Product Type
Product Code
9788120338142_30212
Author
Vennison S. John
Publisher
Phi Learning Pvt Ltd (rs) (rs )
Publication Year
2009
Number Of Pages
124
ISBN-10
8120338146
Languages
English
Publisher Name
Phi Learning Pvt Ltd (rs) (rs )

This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering.The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens.This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology.Key Feature Includes about 60 different experiments.Contains several figures to reinforce the understanding of the techniques discussed.Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices. About the Author S. John Vennison Ph.D., is Lecturer in the Department of Biotechnology, Anna University, Tiruchirappalli. He has more than 10 years of teaching experience and 18 years of research experience in the field of genetic engineering. He has published several research papers in the national and international journals. His research areas include molecular biology and biotechnology. A recipient of the Visiting Scientist Award, 1995 by UNESCO, Dr. Vennison is a lifemember of Biotech Research Society of India. Table of Contents Preface 1. Isolation of Genomic DNA from Gram Negative, Gram Positive Bacteria, Blood and Mammalian Tissue 2. Isolation of Plasmid DNA from Gram Negative and Gram Positive Bacteria 3. Isolation of RNA from Bacteria and Cultured Mammalian Cells 4. Estimation of Nucleic Acids 5. Restriction Digestion and Ligation of DNA 6. Polymerase Chain Reaction and Randomly Amplified Polymorphic DNA 7. Electrophoresis of Nucleic Acids 8. Slot-lysis Agarose Gel Electrophoresis 9. Purification of DNA from Agarose and Polyacrylamide Gels 10. Transformation of Gram Negative and Gram Positive Bacteria with Plasmid DNA 11. Estimation of Proteins 12. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis for Proteins 13. ?-Galactosidase Assay 14. Transduction of Plasmid DNA Using CP?51 and CP?54 Bacteriophages 15. Bacterial Conjugation 16. Blotting Techniques 17. 32P Labelled Probe Preparation and Measurement of Radioactivity in Radiolabelled Nucleic Acid 18. Hybridization Techniques Appendices Index

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